I did this last week. I rode my bike I think twice in about 10 years, then got a bit serious and took about 6 weeks to build up to doing a century. Running endurance translates extremely well for something like that, but as I'm sure you know, running power doesn't. So just take it easy especially for the first 50. By that I mean 1-2mph slower than you'd do on your typical weekend ride.
The one thing that I can't recommend enough is EAT A CRAP TON. This is coming from someone that rarely if ever fuels for 20+ mile runs. I'm a believer in training the body to burn fat vs carbs. But when you're out there for 6 or 7 hours, you have to eat EARLY and often. I had a goal of replacing half the calories of what I burned while riding, and I think that set me up well to finish the ride (104 total) quite comfortably.
I just have an older hybrid as well. I'm sure a decent road bike would have been 1-2mph faster. But it wasn't a race, so no big deal. I finished in just over 6 hours. And again, that's coming from someone who's cycling fitness is awful compared to running fitness. So you can do it.